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odyssey blocking buffer  (LI-COR)


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    Structured Review

    LI-COR odyssey blocking buffer
    Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercept+pbs+blocking+buffer/Intercept+(PBS)+Blocking+Buffer/pm42778065-105-4-7
    Average 98 stars, based on 1115 article reviews
    odyssey blocking buffer - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: TAS3351 is a brain penetrable EGFR-TKI that overcomes T790M and C797S resistant mutations
    Article Snippet: .. After the incubation, the cells were fixed with 20% Formalin Neutral Buffer Solution (FUJIFILM Wako Pure Chemical Corporation), and the wells were washed with phosphate buffered saline (PBS)-Tween 20, followed by treatment with 0.1% Triton-X 100 for membrane permeabilization and with Intercept (PBS) Blocking Buffer (LI-COR) for blocking of nonspecific AR TI CL E IN P RE SS Main Manuscript File, Kasuga et al., binding. .. Both anti-pEGFR (Tyr1068, cat# 2234, Cell Signaling technology) and anti-EGFR (cat# MA5-13697, Thermo Fisher Scientific) antibodies in Interceptor (PBS) Blocking Buffer (1:200 dilution, respectively) were added to the wells and the plates were incubated overnight.

    Article Title: MEK interactions tune RAF kinase sensitivity to conformation-selective inhibition.
    Article Snippet: .. Samples were then incubated with 125 mM glycine in 1× PBS for 5 min, followed by blocking with Intercept PBS blocking buffer (LI-COR Biosciences) for 1 h at 37 °C. .. Then, p44/42 MAPK (ERK1/2) (3A7) mouse mAb (Cell Signaling Technology) and phospho-p44/42 MAPK (ERK1/2) D13.14.4E XP rabbit mAb (Cell Signaling Technology) were diluted in Intercept PBS blocking buffer (LI-COR Biosciences) and added to the samples, followed by incubation for 1 h at room temperature.

    Article Title: Nodavirus protein A’s interdomain elbow controls RNA replication organelle formation and function
    Article Snippet: .. Blots were blocked in Intercept (PBS) Blocking Buffer (LI-COR Biosciences) supplemented with 1% SDS for 1 h at room temperature, then incubated for 30 min at room temperature in a 1:10 000 dilution of IRDye680RD-streptavidin in Intercept (PBS) Blocking Buffer containing 1% SDS. ..

    Article Title: Differentiated SH-SY5Y cells exhibit neuronal features but lack synaptic maturity.
    Article Snippet: .. The gel was then transferred onto a nitrocellulose membrane (Merck) for 70 min at a constant 100 V. The membrane was washed with PBS with 1% Tween (PBST) for 10 min and then incubated with Intercept (PBS) Blocking Buffer (LI-COR Biosciences) in PBST for one hour. ..

    Saline:

    Article Title: TAS3351 is a brain penetrable EGFR-TKI that overcomes T790M and C797S resistant mutations
    Article Snippet: .. After the incubation, the cells were fixed with 20% Formalin Neutral Buffer Solution (FUJIFILM Wako Pure Chemical Corporation), and the wells were washed with phosphate buffered saline (PBS)-Tween 20, followed by treatment with 0.1% Triton-X 100 for membrane permeabilization and with Intercept (PBS) Blocking Buffer (LI-COR) for blocking of nonspecific AR TI CL E IN P RE SS Main Manuscript File, Kasuga et al., binding. .. Both anti-pEGFR (Tyr1068, cat# 2234, Cell Signaling technology) and anti-EGFR (cat# MA5-13697, Thermo Fisher Scientific) antibodies in Interceptor (PBS) Blocking Buffer (1:200 dilution, respectively) were added to the wells and the plates were incubated overnight.

    Article Title: The Drosophila maternal-effect gene abnormal oocyte ( ao ) does not repress histone gene expression
    Article Snippet: We ran the gel for 90 min at 100 V in Tris/Glycine/SDS buffer, then transferred the gel to a Trans-Blot Turbo Mini 0.2 μm Nitrocellulose membrane (Bio-Rad Laboratories) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). .. We washed the membrane 3 times with PBS, blocked in Intercept (PBS) Blocking Buffer (LI-COR) for 1 h at room temperature, then probed with primary antibodies in phosphate-buffered saline with 0.1% Tween-20 (PBST) at 4 °C overnight. .. We used the following primary antibodies: rabbit anti-beta-tubulin (abcam 6046) at 1:1,000, mouse anti-H2B (abcam 52484) at 1:3,000, and rabbit anti-H3 (abcam 1971) at 1:4,000.

    Membrane:

    Article Title: TAS3351 is a brain penetrable EGFR-TKI that overcomes T790M and C797S resistant mutations
    Article Snippet: .. After the incubation, the cells were fixed with 20% Formalin Neutral Buffer Solution (FUJIFILM Wako Pure Chemical Corporation), and the wells were washed with phosphate buffered saline (PBS)-Tween 20, followed by treatment with 0.1% Triton-X 100 for membrane permeabilization and with Intercept (PBS) Blocking Buffer (LI-COR) for blocking of nonspecific AR TI CL E IN P RE SS Main Manuscript File, Kasuga et al., binding. .. Both anti-pEGFR (Tyr1068, cat# 2234, Cell Signaling technology) and anti-EGFR (cat# MA5-13697, Thermo Fisher Scientific) antibodies in Interceptor (PBS) Blocking Buffer (1:200 dilution, respectively) were added to the wells and the plates were incubated overnight.

    Article Title: Comprehensive profiling of clinically approved kinase inhibitors reveals mutation-specific inhibitors and opportunities for drug repurposing.
    Article Snippet: The proteins were transferred to nitrocellulose membrane by a BioRad wet/tank blotting system. .. The membrane was then blocked with LICOR Intercept (PBS) blocking buffer (LICOR, 927-70001) for 1 h at ambient temperature, followed by primary antibody at 1:1,000 in the blocking buffer at 4°C for overnight. .. Primary antibodies used were as follow: β-actin (Sigma-Aldrich, A1978), FLAG tag (Sigma-Aldrich, F1804), N-Myc (Cell Signaling Technology, 51705), S6 ribosomal Protein (Cell Signaling Technology, 2317), phospho-S6 ribosomal protein (S235/236) (Cell Signaling Technology, 2211), phospho-Akt (Cell Signaling Technology, 4058), Akt (Cell Signaling Technology, 2920), phospho-FGFR (Y653/654) (Cell Signaling Technology, 3471), phospho-Met (Y1234/1235) (Cell Signaling Technology, 3077), E-Cadherin (Cell Signaling Technology, 3195), Vimentin (Cell Signaling Technology, 5741) and Slug (Cell Signaling Technology, 9585).

    Article Title: The Drosophila maternal-effect gene abnormal oocyte ( ao ) does not repress histone gene expression
    Article Snippet: We ran the gel for 90 min at 100 V in Tris/Glycine/SDS buffer, then transferred the gel to a Trans-Blot Turbo Mini 0.2 μm Nitrocellulose membrane (Bio-Rad Laboratories) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). .. We washed the membrane 3 times with PBS, blocked in Intercept (PBS) Blocking Buffer (LI-COR) for 1 h at room temperature, then probed with primary antibodies in phosphate-buffered saline with 0.1% Tween-20 (PBST) at 4 °C overnight. .. We used the following primary antibodies: rabbit anti-beta-tubulin (abcam 6046) at 1:1,000, mouse anti-H2B (abcam 52484) at 1:3,000, and rabbit anti-H3 (abcam 1971) at 1:4,000.

    Article Title: Differentiated SH-SY5Y cells exhibit neuronal features but lack synaptic maturity.
    Article Snippet: .. The gel was then transferred onto a nitrocellulose membrane (Merck) for 70 min at a constant 100 V. The membrane was washed with PBS with 1% Tween (PBST) for 10 min and then incubated with Intercept (PBS) Blocking Buffer (LI-COR Biosciences) in PBST for one hour. ..

    Blocking Assay:

    Article Title: TAS3351 is a brain penetrable EGFR-TKI that overcomes T790M and C797S resistant mutations
    Article Snippet: .. After the incubation, the cells were fixed with 20% Formalin Neutral Buffer Solution (FUJIFILM Wako Pure Chemical Corporation), and the wells were washed with phosphate buffered saline (PBS)-Tween 20, followed by treatment with 0.1% Triton-X 100 for membrane permeabilization and with Intercept (PBS) Blocking Buffer (LI-COR) for blocking of nonspecific AR TI CL E IN P RE SS Main Manuscript File, Kasuga et al., binding. .. Both anti-pEGFR (Tyr1068, cat# 2234, Cell Signaling technology) and anti-EGFR (cat# MA5-13697, Thermo Fisher Scientific) antibodies in Interceptor (PBS) Blocking Buffer (1:200 dilution, respectively) were added to the wells and the plates were incubated overnight.

    Article Title: MEK interactions tune RAF kinase sensitivity to conformation-selective inhibition.
    Article Snippet: .. Samples were then incubated with 125 mM glycine in 1× PBS for 5 min, followed by blocking with Intercept PBS blocking buffer (LI-COR Biosciences) for 1 h at 37 °C. .. Then, p44/42 MAPK (ERK1/2) (3A7) mouse mAb (Cell Signaling Technology) and phospho-p44/42 MAPK (ERK1/2) D13.14.4E XP rabbit mAb (Cell Signaling Technology) were diluted in Intercept PBS blocking buffer (LI-COR Biosciences) and added to the samples, followed by incubation for 1 h at room temperature.

    Article Title: Comprehensive profiling of clinically approved kinase inhibitors reveals mutation-specific inhibitors and opportunities for drug repurposing.
    Article Snippet: The proteins were transferred to nitrocellulose membrane by a BioRad wet/tank blotting system. .. The membrane was then blocked with LICOR Intercept (PBS) blocking buffer (LICOR, 927-70001) for 1 h at ambient temperature, followed by primary antibody at 1:1,000 in the blocking buffer at 4°C for overnight. .. Primary antibodies used were as follow: β-actin (Sigma-Aldrich, A1978), FLAG tag (Sigma-Aldrich, F1804), N-Myc (Cell Signaling Technology, 51705), S6 ribosomal Protein (Cell Signaling Technology, 2317), phospho-S6 ribosomal protein (S235/236) (Cell Signaling Technology, 2211), phospho-Akt (Cell Signaling Technology, 4058), Akt (Cell Signaling Technology, 2920), phospho-FGFR (Y653/654) (Cell Signaling Technology, 3471), phospho-Met (Y1234/1235) (Cell Signaling Technology, 3077), E-Cadherin (Cell Signaling Technology, 3195), Vimentin (Cell Signaling Technology, 5741) and Slug (Cell Signaling Technology, 9585).

    Article Title: The Drosophila maternal-effect gene abnormal oocyte ( ao ) does not repress histone gene expression
    Article Snippet: We ran the gel for 90 min at 100 V in Tris/Glycine/SDS buffer, then transferred the gel to a Trans-Blot Turbo Mini 0.2 μm Nitrocellulose membrane (Bio-Rad Laboratories) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). .. We washed the membrane 3 times with PBS, blocked in Intercept (PBS) Blocking Buffer (LI-COR) for 1 h at room temperature, then probed with primary antibodies in phosphate-buffered saline with 0.1% Tween-20 (PBST) at 4 °C overnight. .. We used the following primary antibodies: rabbit anti-beta-tubulin (abcam 6046) at 1:1,000, mouse anti-H2B (abcam 52484) at 1:3,000, and rabbit anti-H3 (abcam 1971) at 1:4,000.

    Article Title: Nodavirus protein A’s interdomain elbow controls RNA replication organelle formation and function
    Article Snippet: .. Blots were blocked in Intercept (PBS) Blocking Buffer (LI-COR Biosciences) supplemented with 1% SDS for 1 h at room temperature, then incubated for 30 min at room temperature in a 1:10 000 dilution of IRDye680RD-streptavidin in Intercept (PBS) Blocking Buffer containing 1% SDS. ..

    Article Title: Biophysical and biochemical studies support PHD inhibitor development as a TPI deficiency therapy.
    Article Snippet: .. The primary antibody solutions consisted of anti-TPI (1:5000, rabbit polyclonal, Invitrogen, Waltham, USA, PIPA521583) and anti-beta-tubulin (1:1000, mouse polyclonal, Developmental Studies Hybridoma Bank, Iowa City, USA, E7-c) diluted in Intercept PBS Blocking Buffer (LiCor BioTech LLC, Lincoln, USA, NC1660556). ..

    Article Title: TLR3 Expression in Villus-Like Enterocytes Drives IFN-III Responses to Enteroviruses
    Article Snippet: 1:200 mouse anti-MUC2 clone 996/1 (Invitrogen), 1:200 rabbit anti-CHGA (Invitrogen), 1:200 mouse anti-Ki67 clone B56 (BD), 1:200 rabbit anti-Lysozyme C (DAKO), 1:400 phalloidin AF-633 (Invitrogen), 1:1000 anti-mouse AF-568 (Invitrogen), and 1:1000 anti-rabbit AF-488 (Invitrogen) were used for staining. .. For western blots, 20 μg of protein lysates were separated on 4-20% polyacrylamide gradient gels (Biorad), transferred to nitrocellulose membranes, blocked with Intercept (PBS) blocking buffer (LICOR), stained overnight with 1:1000 polyclonal anti-MAVS (CST) in blocking buffer & 1 hour in 1:5000 anti-rabbit IRDye680 (LICOR) in blocking buffer, and scanned with an Azure 500 Imager (Azure Biosystems). .. E11 (Gregory) was obtained from the ATCC and propagated on HeLa cells (clone 7B) obtained from Jeff Bergelson, Children’s Hospital of Philadelphia, Philadelphia, PA. E11 was expanded on HeLa cells, concentrated by ultracentrifugation through a sucrose cushion, and titres determined by plaque assay on HeLa cells as described previously ( ).

    Article Title: Differentiated SH-SY5Y cells exhibit neuronal features but lack synaptic maturity.
    Article Snippet: .. The gel was then transferred onto a nitrocellulose membrane (Merck) for 70 min at a constant 100 V. The membrane was washed with PBS with 1% Tween (PBST) for 10 min and then incubated with Intercept (PBS) Blocking Buffer (LI-COR Biosciences) in PBST for one hour. ..

    Binding Assay:

    Article Title: TAS3351 is a brain penetrable EGFR-TKI that overcomes T790M and C797S resistant mutations
    Article Snippet: .. After the incubation, the cells were fixed with 20% Formalin Neutral Buffer Solution (FUJIFILM Wako Pure Chemical Corporation), and the wells were washed with phosphate buffered saline (PBS)-Tween 20, followed by treatment with 0.1% Triton-X 100 for membrane permeabilization and with Intercept (PBS) Blocking Buffer (LI-COR) for blocking of nonspecific AR TI CL E IN P RE SS Main Manuscript File, Kasuga et al., binding. .. Both anti-pEGFR (Tyr1068, cat# 2234, Cell Signaling technology) and anti-EGFR (cat# MA5-13697, Thermo Fisher Scientific) antibodies in Interceptor (PBS) Blocking Buffer (1:200 dilution, respectively) were added to the wells and the plates were incubated overnight.

    Western Blot:

    Article Title: TLR3 Expression in Villus-Like Enterocytes Drives IFN-III Responses to Enteroviruses
    Article Snippet: 1:200 mouse anti-MUC2 clone 996/1 (Invitrogen), 1:200 rabbit anti-CHGA (Invitrogen), 1:200 mouse anti-Ki67 clone B56 (BD), 1:200 rabbit anti-Lysozyme C (DAKO), 1:400 phalloidin AF-633 (Invitrogen), 1:1000 anti-mouse AF-568 (Invitrogen), and 1:1000 anti-rabbit AF-488 (Invitrogen) were used for staining. .. For western blots, 20 μg of protein lysates were separated on 4-20% polyacrylamide gradient gels (Biorad), transferred to nitrocellulose membranes, blocked with Intercept (PBS) blocking buffer (LICOR), stained overnight with 1:1000 polyclonal anti-MAVS (CST) in blocking buffer & 1 hour in 1:5000 anti-rabbit IRDye680 (LICOR) in blocking buffer, and scanned with an Azure 500 Imager (Azure Biosystems). .. E11 (Gregory) was obtained from the ATCC and propagated on HeLa cells (clone 7B) obtained from Jeff Bergelson, Children’s Hospital of Philadelphia, Philadelphia, PA. E11 was expanded on HeLa cells, concentrated by ultracentrifugation through a sucrose cushion, and titres determined by plaque assay on HeLa cells as described previously ( ).

    Staining:

    Article Title: TLR3 Expression in Villus-Like Enterocytes Drives IFN-III Responses to Enteroviruses
    Article Snippet: 1:200 mouse anti-MUC2 clone 996/1 (Invitrogen), 1:200 rabbit anti-CHGA (Invitrogen), 1:200 mouse anti-Ki67 clone B56 (BD), 1:200 rabbit anti-Lysozyme C (DAKO), 1:400 phalloidin AF-633 (Invitrogen), 1:1000 anti-mouse AF-568 (Invitrogen), and 1:1000 anti-rabbit AF-488 (Invitrogen) were used for staining. .. For western blots, 20 μg of protein lysates were separated on 4-20% polyacrylamide gradient gels (Biorad), transferred to nitrocellulose membranes, blocked with Intercept (PBS) blocking buffer (LICOR), stained overnight with 1:1000 polyclonal anti-MAVS (CST) in blocking buffer & 1 hour in 1:5000 anti-rabbit IRDye680 (LICOR) in blocking buffer, and scanned with an Azure 500 Imager (Azure Biosystems). .. E11 (Gregory) was obtained from the ATCC and propagated on HeLa cells (clone 7B) obtained from Jeff Bergelson, Children’s Hospital of Philadelphia, Philadelphia, PA. E11 was expanded on HeLa cells, concentrated by ultracentrifugation through a sucrose cushion, and titres determined by plaque assay on HeLa cells as described previously ( ).



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